Penetration of nanoparticles in flax (Linum usitatissimum L.) calli and regenerants

Inese Kokina; Vjačeslavs Gerbreders; Eriks Sledevskis; Andrejs Bulanovs

doi:10.1016/j.jbiotec.2013.03.011

Penetration of nanoparticles in flax (Linum usitatissimum L.) calli and regenerants

J Biotechnol. 2013 May 20;165(2):127-32. doi: 10.1016/j.jbiotec.2013.03.011. Epub 2013 Mar 29.

Authors

Inese Kokina¹, Vjačeslavs Gerbreders, Eriks Sledevskis, Andrejs Bulanovs

Affiliation

¹ Institute of Systematic Biology, Daugavpils University, Vienības 13, Daugavpils LV-5401, Latvia. inese.kokina@biology.lv

PMID: 23545504
DOI: 10.1016/j.jbiotec.2013.03.011

Abstract

We demonstrate a method for direct delivery of metal nanoparticles to flax calli and regenerant cells by vacuum deposition of metal nanolayers on powdered hormone followed by dispersal of the combined hormone-metal in medium. The penetration and location of the gold (AuNPs) and silver (AgNPs) nanoparticles in calli and in plant regenerants were confirmed by optical absorption spectroscopy and scanning electron microscopy. We detected a significant effect of the AuNPs and AgNPs on the regeneration type of flax calli.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Flax / metabolism*
Gold / chemistry*
Metal Nanoparticles / chemistry*
Metal Nanoparticles / ultrastructure
Microscopy, Electron, Scanning
Silver / chemistry*

Substances

Silver
Gold