An easy, quick and cheap high-throughput method for yeast DNA extraction from microwell plates

J Drumonde-Neves; E Vieira; M T Lima; I Araujo; M Casal; D Schuller

doi:10.1016/j.mimet.2013.03.016

An easy, quick and cheap high-throughput method for yeast DNA extraction from microwell plates

J Microbiol Methods. 2013 Jun;93(3):206-8. doi: 10.1016/j.mimet.2013.03.016. Epub 2013 Mar 29.

Authors

J Drumonde-Neves¹, E Vieira, M T Lima, I Araujo, M Casal, D Schuller

Affiliation

¹ Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Braga 4710-057, Portugal. drumondeneves@gmail.com

PMID: 23545448
DOI: 10.1016/j.mimet.2013.03.016

Abstract

We herein present a high-throughput and cheap method for yeast DNA isolation in a 96 well microplate. About 1500 yeast isolates can be processed within one working day and final DNA concentrations are suitable for direct application in PCR-based molecular typing methods.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Fungal / isolation & purification*
Microbiological Techniques / economics
Microbiological Techniques / methods*
Molecular Biology / economics
Molecular Biology / methods*
Molecular Typing / methods
Mycological Typing Techniques / methods
Specimen Handling / economics
Specimen Handling / methods*
Time Factors
Yeasts / genetics*

Substances

DNA, Fungal