A SRCF cell line from snowtrout, Schizothorax richardsonii: development and characterization

M Goswami; B S Sharma; S N Bahuguna; N S Nagpure; W S Lakra

doi:10.1016/j.tice.2013.02.002

A SRCF cell line from snowtrout, Schizothorax richardsonii: development and characterization

Tissue Cell. 2013 Jun;45(3):219-26. doi: 10.1016/j.tice.2013.02.002. Epub 2013 Mar 29.

Authors

M Goswami¹, B S Sharma, S N Bahuguna, N S Nagpure, W S Lakra

Affiliation

¹ National Bureau of Fish Genetic Resources, Canal Ring Road, PO Dilkusha, Lucknow 226002, India. mukugoswami@gmail.com

PMID: 23545061
DOI: 10.1016/j.tice.2013.02.002

Abstract

Schizothorax richardsonii, commonly called snowtrout, is an important indigenous coldwater fish of the Himalayas, India with high commercial values as food and game fish. A cell line named as SRCF was developed from the caudal fin tissue of S. richardsonii. The cell line has been maintained in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum (FBS) at 24°C. The cells showed fibroblastic morphology, high plating efficiency and cell doubling time of 48h. Chromosomal analysis of SRCF cells revealed a diploid count of 98 chromosomes. The origin of the cell line was confirmed by the amplification of 655 and 578bp of cytochrome oxidase subunit I (COI) and 16S rRNA of mitochondrial DNA (mt-DNA) genes, respectively. Transfection of SRCF cells with pEGFP-C1 plasmid resulted in bright fluorescent signals, suggesting the application of cell line in transgenic and genetic improvement programme. In addition, genotoxicity assessment illustrated the utility of the cell line as an in vitro model for aquatic toxicological studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Fins / cytology*
Animals
Cell Culture Techniques
Cell Line / cytology*
Cyprinidae / growth & development*
Electron Transport Complex IV / genetics
RNA, Ribosomal, 16S / genetics

Substances

RNA, Ribosomal, 16S
Electron Transport Complex IV