Synthesis of fucosyl-N-acetylglucosamine disaccharides by transfucosylation using α-L-fucosidases from Lactobacillus casei

Appl Environ Microbiol. 2013 Jun;79(12):3847-50. doi: 10.1128/AEM.00229-13. Epub 2013 Mar 29.

Abstract

AlfB and AlfC α-l-fucosidases from Lactobacillus casei were used in transglycosylation reactions, and they showed high efficiency in synthesizing fucosyldisaccharides. AlfB and AlfC activities exclusively produced fucosyl-α-1,3-N-acetylglucosamine and fucosyl-α-1,6-N-acetylglucosamine, respectively. The reaction kinetics showed that AlfB can convert 23% p-nitrophenyl-α-l-fucopyranoside into fucosyl-α-1,3-N-acetylglucosamine and AlfC at up to 56% into fucosyl-α-1,6-N-acetylglucosamine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosamine / analogs & derivatives*
  • Acetylglucosamine / biosynthesis
  • Bioreactors*
  • Biotechnology / methods
  • Disaccharides / biosynthesis*
  • Enzyme Stability
  • Kinetics
  • Lacticaseibacillus casei / enzymology*
  • Magnetic Resonance Spectroscopy
  • alpha-L-Fucosidase / metabolism*

Substances

  • Disaccharides
  • fucosyl alpha 1,3 N-acetylglucosamine
  • alpha-L-Fucosidase
  • Acetylglucosamine