Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes

Miguel Angel Merlos Rodrigo; Natalia Cernei; Marketa Kominkova; Ondrej Zitka; Miroslava Beklova; Josef Zehnalek; Rene Kizek; Vojtech Adam

doi:10.3390/ijerph10041304

Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes

Int J Environ Res Public Health. 2013 Mar 28;10(4):1304-11. doi: 10.3390/ijerph10041304.

Authors

Miguel Angel Merlos Rodrigo¹, Natalia Cernei, Marketa Kominkova, Ondrej Zitka, Miroslava Beklova, Josef Zehnalek, Rene Kizek, Vojtech Adam

Affiliation

¹ Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. miguelangel.merlos@eez.csic.es

Abstract

In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 µg·mL(-1) PC2 and 100 µg·mL(-1) of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cadmium / chemistry*
Chromatography, Ion Exchange / methods*
Environmental Monitoring / methods
Environmental Pollutants / chemistry*
Phytochelatins / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Environmental Pollutants
Cadmium
Phytochelatins