The aims of this study were to compare the chondroitin sulphate (CS) levels in gingival crevicular fluid (GCF) of moved canines using either 70 or 120 g of orthodontic force, and to compare the rate of tooth movement and the amount of pain between these two force magnitudes. Sixteen patients (6 males and 10 females; aged 16.91 ± 2.99 years), with class I malocclusion, who required orthodontic treatment with first premolar extractions, were recruited. The force magnitudes used to move the maxillary canines distally were controlled at 70 and 120 g on the right and the left sides, respectively. GCF samples were collected with Periopaper(®) strips before and during orthodontic tooth movement. Competitive ELISA with monoclonal antibody was used to measure the CS levels. The distance of tooth movement and the amount of pain assessed by visual analog scale (VAS) scores were evaluated. The medians of CS levels during the loaded period were significantly greater than those during the unloaded period (P < 0.05). The differences between the medians of CS levels of 70 g and 120 g retraction force during each 1 week period were not significant. There was no significant difference in the rates of canine movement between these two force magnitudes. However, using 120 g, the medians of VAS scores were significantly greater than those with 70 g (P < 0.05). Collectively, 70 g retraction force appears to be sufficient and more suitable than 120 g force as it causes no difference in biochemically-assessed bone remodelling activity, the same rate of tooth movement, reduced pain and better comfort.