Mimicking nanofibrous hybrid bone substitute for mesenchymal stem cells differentiation into osteogenesis

Macromol Biosci. 2013 Jun;13(6):696-706. doi: 10.1002/mabi.201200435. Epub 2013 Mar 25.

Abstract

Mimicking hybrid extracellular matrix is one of the main challenges for bone tissue engineering (BTE). Biocompatible polycaprolactone/poly(α,β)-DL-aspartic acid/collagen nanofibrous scaffolds were fabricated by electrospinning and nanohydroxyapatite (n-HA) was deposited by calcium phosphate dipping method for BTE. Human mesenchymal stem cells (hMSCs) were cultured on these hybrid scaffolds to investigate the cell proliferation, osteogenic differentiation by alkaline phosphatase activity, mineralization, double immunofluorescent staining using CD90 and expression of osteocalcin. The present study indicated that the PCL/PAA/collagen/n-HA scaffolds promoted greater osteogenic differentiation of hMSCs, proving to be a potential hybrid scaffolds for BTE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Bone Substitutes / pharmacology*
  • Calcification, Physiologic / drug effects
  • Cell Adhesion / drug effects
  • Cell Differentiation / drug effects*
  • Cell Proliferation / drug effects
  • Collagen / pharmacology
  • Durapatite / pharmacology
  • Humans
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / enzymology
  • Mesenchymal Stem Cells / ultrastructure
  • Nanofibers / chemistry*
  • Nanofibers / ultrastructure
  • Osteocalcin / metabolism
  • Osteogenesis / drug effects*
  • Peptides / pharmacology
  • Polyesters / pharmacology
  • Porosity
  • Spectroscopy, Fourier Transform Infrared
  • Tensile Strength / drug effects
  • Tissue Scaffolds / chemistry
  • Water

Substances

  • Bone Substitutes
  • Peptides
  • Polyesters
  • nano-hydroxyapatite-collagen
  • Water
  • Osteocalcin
  • polycaprolactone
  • polyaspartate
  • Collagen
  • Durapatite
  • Alkaline Phosphatase