A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons

PLoS One. 2013;8(3):e58741. doi: 10.1371/journal.pone.0058741. Epub 2013 Mar 14.

Abstract

Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows specific detection of atypical Chlamydiaceae from pigeons. The assay has been used to assess the dissemination of these strains in field samples collected from Parisian pigeon populations in 2009. The results suggest a limited dissemination compared to the usually higher prevalence of Chlamydia psittaci that is the main species associated with avian chlamydiosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chlamydiaceae / classification
  • Chlamydiaceae / enzymology
  • Chlamydiaceae / genetics*
  • Chlamydiaceae / isolation & purification*
  • Columbidae / microbiology*
  • DNA, Bacterial / genetics
  • Genome, Bacterial / genetics
  • Phosphopyruvate Hydratase / genetics
  • Phylogeny
  • Real-Time Polymerase Chain Reaction / methods*
  • Reproducibility of Results

Substances

  • DNA, Bacterial
  • Phosphopyruvate Hydratase

Grants and funding

This work was supported by grants from Region Ile-de-France (post-doctoral fellowship and Sustainable Development 145 Network R2DS, No. 2008-07). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.