From the methanolic extract of Eysenhardtia platycarpa (leaves), were isolated the prenylated flavanones: 5,7-dihydroxy-6-methyl-8-prenylflavanone (1), 5,7-dihydroxy-6-methyl-8-prenyl-4'-methoxy-flavanone (2), 5,7-dihydroxy-6-prenylflavanone (3), 5-dihydroxy-7-methoxy-6-prenylflavanone (4), 5,7-dihydroxy-8-prenyl-4'-methoxy-flavanone (5). Methanolic extract of E. platycarpa (leaves) and flavanones (5), (2) and (3) showed anti-inflammatory activity according to tested quantities. In addition, flavanone (5) revealed the best percentage reduction of free radical DPPH. Finally, after cytotoxic activity study, the methanolic extract from E. platycarpa leaves and flavanone (4) exhibited promising cytotoxic activity on brine shrimp.