Abstract
We introduced a positive feedback loop into a LacI-dependent gene expression system in lipid vesicles, producing a cell-like system that senses and responds to an external signal with a high signal-to-noise ratio. This fully reconstituted system will be a useful tool in future applications in in vitro synthetic biology.
MeSH terms
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Biosensing Techniques*
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Escherichia coli / genetics*
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Escherichia coli Proteins / genetics
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Feedback, Physiological
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Gene Expression*
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Genetic Engineering
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Isopropyl Thiogalactoside / chemistry
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Lac Repressors / genetics
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Liposomes / metabolism*
Substances
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Escherichia coli Proteins
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Lac Repressors
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LacI protein, E coli
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Liposomes
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Isopropyl Thiogalactoside