Abstract
Background:
The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats.
Results:
Cisplatin increased GC apoptosis from 0.59% to 13.04% in the control and cisplatin treatment groups, respectively, which was significantly reduced upon co-culture with BMSCs to 4.84%. Cisplatin treatment increased p21 and bax and decreased c-myc mRNA expression, which was reversed upon co-culture with BMSCs. As compared to young rats, increased apoptosis was observed in the perimenopausal rats (P < 0.001). After 3 months, the apoptosis rate in the BMSC group was significantly lower than that of the control group (P = 0.007).
Conclusions:
BMSC therapy may protect against GC apoptosis induced by cisplatin and perimenopause. Further studies are necessary to evaluate therapeutic efficacy of BMSCs.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Apoptosis / drug effects
-
Bone Marrow Cells / drug effects*
-
Bone Marrow Cells / physiology
-
Cells, Cultured
-
Cisplatin / administration & dosage*
-
Coculture Techniques / methods*
-
Cyclin-Dependent Kinase Inhibitor p21 / genetics
-
Cyclin-Dependent Kinase Inhibitor p21 / metabolism*
-
Estrogens / administration & dosage
-
Female
-
Gene Expression Regulation / drug effects
-
Granulosa Cells / drug effects*
-
Granulosa Cells / physiology
-
Mesenchymal Stem Cell Transplantation*
-
Mesenchymal Stem Cells / drug effects*
-
Mesenchymal Stem Cells / physiology
-
Perimenopause / physiology*
-
Proto-Oncogene Proteins c-myc / genetics
-
Proto-Oncogene Proteins c-myc / metabolism
-
Rats
-
Rats, Sprague-Dawley
-
bcl-2-Associated X Protein / genetics
-
bcl-2-Associated X Protein / metabolism
Substances
-
Cyclin-Dependent Kinase Inhibitor p21
-
Estrogens
-
Proto-Oncogene Proteins c-myc
-
bcl-2-Associated X Protein
-
Cisplatin