The hydrodynamic characteristics of UASB bioreactors operated under different organic loading and hydraulic loading rates were studied, using three laboratory scale models treating concocted sucrose wastewater. Residence time distribution (RTD) analysis using dispersion model and tanks-in-series model was directed towards the characterization of the fluid flow pattern in the reactors and correlation of the hydraulic regime with the biomass content and biogas production. Empty bed reactors followed a plug flow pattern and the flow pattern changed to a large dispersion mixing with biomass and gas production. Effect of increase in gas production on the overall hydraulics was insignificant.