Abstract
In Neurospora crassa, unpaired genes are silenced by a mechanism called meiotic silencing by unpaired DNA (MSUD). Although some RNA interference proteins are necessary for this process, its requirement of small RNAs has yet to be formally established. Here we report the characterization of small RNAs targeting an unpaired region, using Illumina sequencing.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Base Sequence
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Crosses, Genetic
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DNA, Fungal / metabolism*
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Exons / genetics
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GC Rich Sequence / genetics
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Gene Silencing*
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Genes, Fungal
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Genetic Loci / genetics
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Introns / genetics
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Meiosis / genetics*
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Neurospora crassa / cytology*
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Neurospora crassa / genetics*
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Nucleotides / genetics
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RNA, Fungal / metabolism*
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RNA, Small Interfering / metabolism
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Reproducibility of Results
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Spores, Fungal / genetics
Substances
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DNA, Fungal
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Nucleotides
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RNA, Fungal
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RNA, Small Interfering