Selection of stable reference genes in heat stressed peripheral blood mononuclear cells of tropically adapted Indian cattle and buffaloes

Amit Kishore; Monika Sodhi; Keviletsu Khate; Neha Kapila; Parvesh Kumari; Manishi Mukesh

doi:10.1016/j.mcp.2013.02.003

Selection of stable reference genes in heat stressed peripheral blood mononuclear cells of tropically adapted Indian cattle and buffaloes

Mol Cell Probes. 2013 Jun-Aug;27(3-4):140-4. doi: 10.1016/j.mcp.2013.02.003. Epub 2013 Mar 14.

Authors

Amit Kishore¹, Monika Sodhi, Keviletsu Khate, Neha Kapila, Parvesh Kumari, Manishi Mukesh

Affiliation

¹ National Bureau of Animal Genetic Resources, Karnal, Haryana 132001, India.

PMID: 23499765
DOI: 10.1016/j.mcp.2013.02.003

Abstract

Eleven reference genes from different functional categories were assessed for their stable expression pattern in heat stressed PBMCs across Indian buffalo and cattle using GeNorm, NormFinder and BestKeeper algorithms. As the first report, we suggest B2M, RPS9 and RPS15a as suitable reference genes for accurate normalization of PBMC transcript data while the ACTB gene is not recommended.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms*
Animals
Buffaloes / genetics*
Cattle / genetics*
Gene Expression Profiling
Heat-Shock Proteins / genetics*
Leukocytes, Mononuclear / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tropical Climate

Substances

Heat-Shock Proteins