Changes of intestinal tight junctions in rat ileums after partial hepatectomy

Transplant Proc. 2013 Mar;45(2):649-53. doi: 10.1016/j.transproceed.2012.02.052.

Abstract

Background: Increased intestinal permeability and bacterial translocation have been observed after partial hepatectomy (PH).

Methods: Rats (n = 40) were randomly divided into four groups: control; PH 1d, PH 3d and PH 5d. PH animals underwent two-thirds partial hepatectomy before sacrifice at 1, 3, or 5 days thereafter. The intestinal tight junction (TJ) morphology was observed by transmission electron microscopy. Distribution of TJ protein occludin in lipid raft microdomains was examined using Western blot analysis.

Results: Intestinal TJ structures revealed significant changes at 1 day after PH that had partly recovered by 5 days. During this period, occludin protein decreased significantly in lipid raft microdomains, increasing partially by 5 days.

Conclusion: PH induced disruption of TJ structures in rat ilea, which was associated with redistribution of occludin protein in lipid raft microdomains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Hepatectomy / methods*
  • Ileum / metabolism*
  • Ileum / ultrastructure
  • Male
  • Membrane Microdomains / metabolism
  • Membrane Microdomains / ultrastructure
  • Microscopy, Electron, Transmission
  • Occludin / metabolism
  • Permeability
  • Rats
  • Rats, Sprague-Dawley
  • Tight Junctions / metabolism*
  • Tight Junctions / ultrastructure
  • Time Factors

Substances

  • Occludin
  • Ocln protein, rat