Inhibitory effects of maleimide derivatives from the mycelia of the fungus Antrodia cinnamomea BCRC 36799 on nitric oxide production in lipopolysaccharide (LPS)-activated RAW264.7 macrophages

Chem Biodivers. 2013 Mar;10(3):434-41. doi: 10.1002/cbdv.201200258.

Abstract

Four new maleimide derivatives, antrocinnamomins E-H (1-4, resp.), together with (3S,4R)-1-hydroxy-3-(4-hydroxyphenyl)-4-(2-methylpropyl)pyrrolidine-2,5-dione (5) and ergosterol were isolated from the mycelia of Antrodia cinnamomea BCRC 36799. The structures were elucidated by 1D- and 2D-NMR spectroscopy, and mass spectrometry. Compounds 1-5 were evaluated for their inhibitory effects on nitric oxide (NO) production by macrophages. Compounds 2 and 4 showed stronger inhibition of NO production than the positive control quercetin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antrodia / chemistry*
  • Cell Line
  • Lipopolysaccharides / toxicity
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Magnetic Resonance Spectroscopy
  • Maleimides / chemistry*
  • Maleimides / isolation & purification
  • Maleimides / pharmacology
  • Mice
  • Molecular Conformation
  • Mycelium / chemistry
  • Nitric Oxide / metabolism*
  • Quercetin / chemistry
  • Quercetin / pharmacology

Substances

  • Lipopolysaccharides
  • Maleimides
  • maleimide
  • Nitric Oxide
  • Quercetin