Problem-solving test: pyrosequencing

Biochem Mol Biol Educ. 2013 Mar-Apr;41(2):112-5. doi: 10.1002/bmb.20688. Epub 2013 Mar 12.

Abstract

Terms to be familiar with before you start to solve the test: Maxam-Gilbert sequencing, Sanger sequencing, gel electrophoresis, DNA synthesis reaction, polymerase chain reaction, template, primer, DNA polymerase, deoxyribonucleoside triphosphates, orthophosphate, pyrophosphate, nucleoside monophosphates, luminescence, acid anhydride bond, phosphodiester bond, mismatch, proofreading, 5'→3' exonuclease.

MeSH terms

  • Educational Measurement*
  • Sequence Analysis, DNA / methods*
  • Surveys and Questionnaires
  • Temperature*