Abstract
Five new eunicellin-based diterpenoids, krempfielins E-I (1-5) and seven known compounds (6-12) were isolated from the organic extract of a Taiwanese soft coral Cladiella krempfi. The structures of the new metabolites were elucidated on the basis of extensive spectroscopic analysis. Metabolites 5, 6, 10 and 12 were shown to exhibit cytotoxicity against a limited panel of cancer cell lines. Furthermore, compounds 6 and 10 could potently inhibit the accumulation of the pro-inflammatory iNOS protein, and 6 and 12 could significantly reduce the expression of COX-2 protein in LPS-stimulated RAW264.7 macrophage cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anthozoa / metabolism*
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology*
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Cell Line, Tumor
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Cyclooxygenase 2 / metabolism
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Diterpenes / chemistry
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Diterpenes / isolation & purification
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Diterpenes / pharmacology*
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Humans
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Inflammation / drug therapy
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Inflammation / pathology
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / pathology
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Mice
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Neoplasms / drug therapy
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Neoplasms / pathology
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Nitric Oxide Synthase Type II / metabolism
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Spectrum Analysis
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Taiwan
Substances
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Anti-Inflammatory Agents
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Antineoplastic Agents
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Diterpenes
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Lipopolysaccharides
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eunicellin
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2