Abstract
Previous studies have shown that the 3' UTR of BmVMP23 was destroyed by an inserted fragment, and the BmVMP23 expression was downregulated in the lethal egg of "Ming" (l-e(m)). In this study, we found a miRNA (bmo-miR-1a-3p) that matches the 3' UTR sequence of BmVMP23 perfectly. The relationship between bmo-miR-1a-3p and the BmVMP23 expression was examined by the co-transfection in vitro. The luciferase assay result showed that luc expression was strongly repressed. This result inferred that bmo-miR-1a-3p may downregulate BmVMP23 expression via complementary interaction with the target sites at the 3' UTR.
Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / genetics
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Animals
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Base Sequence
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Bombyx / cytology
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Bombyx / genetics*
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Bombyx / metabolism
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Cell Line
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Egg Proteins / genetics*
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Egg Proteins / metabolism
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Female
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Gene Expression Regulation*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Insect Proteins / genetics*
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Insect Proteins / metabolism
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Luciferases / genetics
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Luciferases / metabolism
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MicroRNAs / genetics*
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MicroRNAs / metabolism
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Microscopy, Fluorescence
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Molecular Sequence Data
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Mutation
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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3' Untranslated Regions
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Egg Proteins
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Insect Proteins
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MicroRNAs
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vitelline membrane proteins
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Green Fluorescent Proteins
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Luciferases