[Comparison of anti-inflammatory effect and analysis of astilbin red and white transverse section Smilax glabra in 28 collection sites]

Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(23):3595-8.
[Article in Chinese]

Abstract

Objective: Astilbin in 28 Smilax glabra (red and white cross-section) from different sources was determined by HPLC. Pharmacodynamics and component of S. glabra was investigated through inflammation experiment (penetration type).

Method: The analysis was performed on a Hypersil ODS2 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0. 1% acetic acid aqueous (21: 79) at a flow rate of 1.0 mL x min(-1). The detection wavelength was 291 nm, and the column temperature was 25 degrees C. Anti-inflammatory effect was compared from two type cross-section of Smilax glabra in capillary permeability experiment.

Result: Linear correlation was good in the range of 0.003 379-4.004 microg, and the average recoveries were 100.1%, 101.9%, 99.3%, respectively. The content of astilbin in white and red transverse section were 0.19%-2.46% and 2.10%-5.92%, respectively. Anti-inflammatory efficiency of sectioned red and white were were 21% and 32%, respectively.

Conclusion: Astilbin content and anti-inflammatory effect is significantly different between red and white transverse section of S. glabra, the content of astilbin is not positively related with anti-inflammatory effect.

Publication types

  • Comparative Study
  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry*
  • Anti-Inflammatory Agents / pharmacology
  • China
  • Chromatography, High Pressure Liquid
  • Drugs, Chinese Herbal / chemistry*
  • Drugs, Chinese Herbal / pharmacology
  • Female
  • Flavonols / chemistry*
  • Flavonols / pharmacology
  • Male
  • Mice
  • Permeability
  • Smilax / chemistry*

Substances

  • Anti-Inflammatory Agents
  • Drugs, Chinese Herbal
  • Flavonols
  • astilbin