A large scale molecular study of Giardia duodenalis in horses from Colombia

Vet Parasitol. 2013 Sep 1;196(1-2):31-6. doi: 10.1016/j.vetpar.2013.02.006. Epub 2013 Feb 21.

Abstract

The prevalence of Giardia duodenalis assemblages in horses is poorly known. The present study examined feces from 195 horses, 1 month-17 years of age, in 4 locations in Colombia. Prevalence of infection was determined by PCR and all positives were sequenced to determine the genotypes. Thirty four (17.4%) horses were found positive. This is the first report of G. duodenalis in horses from Colombia. Prevalence in female and male horses was 18.9% and 15.1%, respectively. Prevalence in horses <1 year of age and horses >1 year of age was 21.1% and 15.1%, respectively. Molecular characterization using the beta giardin (bg), glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), and small subunit ribosomal RNA (ssurRNA) genes identified G. duodenalis Assemblages A and B, the assemblages regarded as zoonotic.

Keywords: Assemblage; Genotype; Giardia duodenalis; Horse; PCR; Prevalence.

MeSH terms

  • Aging
  • Animals
  • Colombia / epidemiology
  • Feces / parasitology
  • Female
  • Giardia lamblia / genetics*
  • Giardiasis / epidemiology
  • Giardiasis / parasitology
  • Giardiasis / veterinary*
  • Horse Diseases / epidemiology
  • Horse Diseases / parasitology*
  • Horses
  • Male
  • Prevalence