Glucosamine-conjugated nanoparticles for the separation of insulin-secreting beta cells

Adv Healthc Mater. 2013 Sep;2(9):1198-203. doi: 10.1002/adhm.201200479. Epub 2013 Mar 8.

Abstract

A small molecule, glucosamine, is used as targeting moiety for insulin-secreting beta cell separation in artificial cell mixtures and tissue samples. The specificity of glucosamine allows it to be used in cell sorting applications. In addition, a thrombin-specific cleavable peptide was used as an intermediary to release nanoparticles from cell surfaces to facilitate cell attachment and proliferation.

Keywords: beta cell separation; glucosamine; iron oxide nanoparticles; quantum dots.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Separation / methods*
  • Fibroblasts / cytology
  • Fluorescent Dyes / chemistry
  • Glucosamine / chemistry*
  • Glucosamine / metabolism
  • Glucose Transporter Type 2 / metabolism
  • Insulin-Secreting Cells / cytology*
  • Mice
  • Nanoparticles / chemistry*
  • Peptides / chemistry
  • Peptides / metabolism
  • Protein Binding
  • Quantum Dots / chemistry

Substances

  • Fluorescent Dyes
  • Glucose Transporter Type 2
  • Peptides
  • Glucosamine