Abstract
Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC₅₀ (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 mg mL⁻¹ and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 mg GAE g⁻¹ of dry extract. Fe²⁺-induced lipid peroxidation was significantly reduced by the ethanolic extract and fractions. Mitochondrial Ca²⁺-induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in a concentration-dependent manner. Ethanolic extract reduced mitochondrial Δψm only at high concentrations (40-100 mg mL⁻¹), which indicates that its toxicity does not overlap with its antioxidant effects. Results suggest involvement of antioxidant activities of T. catigua in its pharmacological properties.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / analysis
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Antioxidants / chemistry
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Antioxidants / isolation & purification
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Antioxidants / pharmacology*
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Brain / drug effects
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Brain / metabolism
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Brazil
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Ethnopharmacology
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Flavonoids / analysis
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Flavonoids / pharmacology
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Lipid Peroxidation / drug effects
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Male
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Meliaceae / chemistry*
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Membrane Potential, Mitochondrial / drug effects
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Mitochondria, Liver / drug effects
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Mitochondria, Liver / metabolism
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Osmolar Concentration
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Phenols / analysis
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Phenols / pharmacology
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Plant Bark / chemistry*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plant Stems / chemistry*
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Rats
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Rats, Wistar
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Reactive Oxygen Species / antagonists & inhibitors
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Reactive Oxygen Species / metabolism
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Solvents / chemistry
Substances
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Antioxidants
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Flavonoids
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Phenols
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Plant Extracts
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Reactive Oxygen Species
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Solvents