Evaluation of the safety of ancient strains of wheat in coeliac disease reveals heterogeneous small intestinal T cell responses suggestive of coeliac toxicity

Abstract

Background & aims: Coeliac disease is a chronic small intestinal immune-mediated enteropathy triggered by dietary gluten in genetically predisposed individuals. Since it is unknown if all wheat varieties are equally toxic to coeliac patients seven Triticum accessions showing different origin (ancient/modern) and ploidy (di-, tetra- hexaploid) were studied.

Materials and methods: Selected strains of wheat were ancient Triticum monococcum precoce (AA genome) and Triticum speltoides (BB genome), accessions of Triticum turgidum durum (AABB genome) including two ancient (Graziella Ra and Kamut) and two modern (Senatore Cappelli and Svevo) durum strains of wheat and Triticum aestivum compactum (AABBDD genome). Small intestinal gluten-specific T-cell lines generated from 13 coeliac patients were tested with wheat accessions by proliferation assays.

Results: All strains of wheat independent of ploidy or ancient/modern origin triggered heterogeneous responses covering wide ranges of stimulation indices.

Conclusion: Ancient strains of wheat, although previously suggested to be low or devoid of coeliac toxicity, should be tested for immunogenicity using gluten-specific T-cell lines from multiple coeliac patients rather than gluten-specific clones to assess their potential toxicity. Our findings provide further evidence for the need for a strict gluten-free diet in coeliac patients, including avoidance of ancient strains of wheat.

Keywords: Ancient strains of wheat; CD; Coeliac disease; GFD; GR_AABB; Heterogenous T-cell responses; IEL; K_AABB; PTG; Proliferation assays; SC_AABB; SI; S_AABB; Small intestinal gluten-sensitive T-cell lines; T-cell line; TCL; Tac_AABBDD; Tmp_AA; Triticum aestivum compactum (AABBDD genome); Triticum monococcum precoce (AA genome); Triticum speltoides (BB genome); Triticum turgidum durum Graziella Ra (AABB genome); Triticum turgidum durum Kamut (AABB genome); Triticum turgidum durum Senatore Cappelli (AABB genome); Triticum turgidum durum Svevo (AABB genome); Ts_BB; coeliac disease; gluten-free diet; intraepithelial lymphocytes; peptic-tryptic digest of gluten; stimulation index; tTG; tissue transglutaminase.