Biotransformation of 14-deoxy-14-methylenetriptolide into a novel hydroxylation product by Neurospora crassa

Zhouzhou Chen; Ji'an Li; Huimin Lin; Lei Shao; Jihong Qin; Xing Fan; Xiaojing Dong; Daijie Chen

doi:10.1016/j.jbiosc.2013.01.023

Biotransformation of 14-deoxy-14-methylenetriptolide into a novel hydroxylation product by Neurospora crassa

J Biosci Bioeng. 2013 Aug;116(2):199-202. doi: 10.1016/j.jbiosc.2013.01.023. Epub 2013 Mar 5.

Authors

Zhouzhou Chen¹, Ji'an Li, Huimin Lin, Lei Shao, Jihong Qin, Xing Fan, Xiaojing Dong, Daijie Chen

Affiliation

¹ The State Key Lab of New Drug and Pharmaceutical Processes, Shanghai Institute of Pharmaceutical Industry, 1320 West Beijing Rd, Shanghai 200040, China.

PMID: 23465510
DOI: 10.1016/j.jbiosc.2013.01.023

Abstract

The biotransformation of 14-deoxy-14-methylenetriptolide by Neurospora crassa CGMCC AS 3.1604 to produce a new hydroxylation derivative was studied. The structure of this novel compound was determined using spectral data. This biotransformation using whole cells conditioned for 4 days transformed approximately 65% (mol ratio) of the substrate into the compound (5R)-5-hydroxy-14-deoxy-14-methylenetriptolide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotransformation
Diterpenes / chemistry
Diterpenes / metabolism*
Epoxy Compounds / chemistry
Epoxy Compounds / metabolism*
Hydroxylation
Neurospora crassa / metabolism*

Substances

(5R)-5-hydroxy-14-deoxy-14-methylenetriptolide
14-deoxy-14-methylenetriptolide
Diterpenes
Epoxy Compounds