Abstract
This study examined the effect of pinacidil, a nonselective adenosine triphosphate-sensitive potassium channel opener, on the function of osteoblastic MC3T3-E1 cells. Pinacidil caused a significant elevation of collagen synthesis, alkaline phosphatase activity, osteocalcin synthesis and mineralization in the cells (p < 0.05). Pinacidil significantly decreased the production of osteoclast differentiation inducing factors such as TNF-α, IL-6 and receptor activator of nuclear factor-κB ligand in the presence of antimycin A, which inhibits mitochondrial electron transport. Moreover, pinacidil prevented antimycin A-induced reactive oxygen species and nitrotyrosine production. These results demonstrate that pinacidil may have positive effects on skeletal structure.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Alkaline Phosphatase / metabolism
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Animals
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Antimycin A / pharmacology
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Calcium / metabolism
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Cell Differentiation / drug effects*
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Collagen / biosynthesis
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Cytokines / immunology
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Dose-Response Relationship, Drug
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Ion Channel Gating / drug effects
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Mice
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Osteoblasts / drug effects*
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Osteoblasts / physiology*
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Osteocalcin / biosynthesis
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Pinacidil / pharmacology*
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Potassium Channels / metabolism*
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RANK Ligand / metabolism
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Reactive Oxygen Species / metabolism
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Tyrosine / analogs & derivatives
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Tyrosine / metabolism
Substances
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Cytokines
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Potassium Channels
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RANK Ligand
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Reactive Oxygen Species
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Tnfsf11 protein, mouse
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Osteocalcin
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3-nitrotyrosine
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Tyrosine
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Antimycin A
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Pinacidil
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Collagen
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Alkaline Phosphatase
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Calcium