We carried out a cross-sectional study to determine the level of knowledge and awareness regarding children's food safety issues among the school-based street food vendors in Dhaka city. A total of 250 school-based street food vendors were interviewed employing a pre-tested structured questionnaire comprising foodborne illness and food hygiene-related questions. We used a scoring system based on the responses obtained from them, and categorized the overall level of knowledge and awareness into "adequate" and "inadequate." Multivariable logistic regression was used to explore the association between selected sociodemographic characteristics and the level of knowledge and awareness. The most common food item vended by school-based street food vendors was chotpoti/fuchka (37.2%). The median number of schoolchildren customers was 120 per vendor per day. All (100%) vendors were male with a mean age of 30.95±8.8 years, and their mean daily income was 131.16±62.54 Bangladeshi Taka (1.97±0.94 USD). Most (40.1%) of the respondents belonged to the age group 25-34 years, and the majority (43.6%) did not have any formal education. More than two-thirds (68%) vendors could not show adequate level of knowledge and awareness of children's food safety issues. The most common source of obtaining food safety information by vendors was electronic media (91.8%). Elderly (≥45 years) vendors were 17.73 times more likely to have adequate level of knowledge and awareness than the vendors belonging to age group 15-24 years (p<0.001; adjusted OR=17.73; 95% CI=4.38-71.73). Individuals who had an education of higher than primary level were 9.87 times more likely to possess adequate level of knowledge and awareness than those who did not have any formal education (p<0.01; adjusted OR=9.87; 95% CI=2.07-46.93). The majority of school-based street food vendors showed an inadequate level of knowledge and awareness of children's food safety issues.