Abstract
Patients may harbor both drug-susceptible and -resistant bacteria, representing heteroresistance. We studied mixtures of isoniazid-resistant and -susceptible Mycobacterium tuberculosis strains. Conventional drug susceptibility testing was the most sensitive method of detection, whereas the line probe assay and sequencing were not able to detect the clinically relevant 1% proportion of resistant bacteria.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antitubercular Agents / pharmacology*
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Antitubercular Agents / therapeutic use
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Bacterial Proteins / genetics
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Base Sequence
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Catalase / genetics
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Drug Resistance, Bacterial / genetics*
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Genotype
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Humans
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Isoniazid / pharmacology*
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Isoniazid / therapeutic use
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Microbial Sensitivity Tests
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Mycobacterium tuberculosis / drug effects*
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Mycobacterium tuberculosis / genetics
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Oxidoreductases / genetics
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Polymerase Chain Reaction
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Sequence Analysis, DNA
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Tuberculosis, Multidrug-Resistant / drug therapy
Substances
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Antitubercular Agents
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Bacterial Proteins
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Oxidoreductases
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Catalase
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katG protein, Mycobacterium tuberculosis
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InhA protein, Mycobacterium
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Isoniazid