Functional living biointerphases

Adv Healthc Mater. 2013 Sep;2(9):1213-8. doi: 10.1002/adhm.201200473. Epub 2013 Feb 28.

Abstract

Lactococcus lactis is modified to express a fibronectin fragment (FNIII₇₋₁₀) as a membrane protein. This interphase, based on a living system, can be further exploited to provide spatio-temporal factors to direct cell function at the material interface. This approach establishes a new paradigm in biomaterial surface functionalization for biomedical applications.

Keywords: bacteria; cell adhesion; fibronectin; integrin; interface.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Line
  • Cell Survival
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Fibronectins / chemistry
  • Fibronectins / genetics
  • Fibronectins / metabolism*
  • Focal Adhesion Protein-Tyrosine Kinases / metabolism
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Lactococcus lactis / metabolism*
  • Mice
  • Microscopy, Confocal
  • Protein Binding
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Signal Transduction
  • Surface Properties

Substances

  • Fibronectins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Focal Adhesion Protein-Tyrosine Kinases