Endonuclease IV discriminates mismatches next to the apurinic/apyrimidinic site in DNA strands: constructing DNA sensing platforms with extremely high selectivity

Xianjin Xiao; Yang Liu; Meiping Zhao

doi:10.1039/c3cc40902c

Endonuclease IV discriminates mismatches next to the apurinic/apyrimidinic site in DNA strands: constructing DNA sensing platforms with extremely high selectivity

Chem Commun (Camb). 2013 Apr 7;49(27):2819-21. doi: 10.1039/c3cc40902c.

Authors

Xianjin Xiao¹, Yang Liu, Meiping Zhao

Affiliation

¹ Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.

PMID: 23443241
DOI: 10.1039/c3cc40902c

Abstract

A unique capability of Endonuclease IV in discrimination of mismatches neighboring a natural abasic site in DNA strands has been demonstrated, which enables genotyping of SNPs with high discrimination factors and differentiation of as low as 0.1-0.01% of target DNA strands from a large background of single-base different interfering strands.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apurinic Acid / chemistry*
Apurinic Acid / metabolism
Binding Sites
Biosensing Techniques*
DNA / chemistry
DNA / genetics*
DNA-(Apurinic or Apyrimidinic Site) Lyase / metabolism
Deoxyribonuclease IV (Phage T4-Induced) / metabolism*
Genotype
Humans
Kinetics
Polymorphism, Single Nucleotide / genetics*
Polynucleotides / chemistry*
Polynucleotides / metabolism
Substrate Specificity

Substances

Polynucleotides
apyrimidinic acid
Apurinic Acid
DNA
Deoxyribonuclease IV (Phage T4-Induced)
DNA-(Apurinic or Apyrimidinic Site) Lyase