A novel reusable PAni-PVA-Amylase film: activity and analysis

Colloids Surf B Biointerfaces. 2013 Jun 1:106:46-50. doi: 10.1016/j.colsurfb.2013.01.031. Epub 2013 Jan 23.

Abstract

Alpha-amylase was immobilized onto polyaniline-polyvinyl alcohol (PAni-PVA) film by cross linking with glutaraldehyde. The activity yield and immobilization efficiency was calculated as 13.58% and 30.60% respectively. The PAni-PVA-Amylase films were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and amylase activity assay. The immobilized α-amylase had its optimum activity at pH 7.0 and 25°C. The immobilization of α-amylase onto the PAni-PVA films protected the enzyme from Fe(2+) inhibition. The PAni-PVA-Amylase films could be used effectively for 30 cycles with 60% retention of the activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds / chemistry*
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism*
  • Equipment Reuse*
  • Glutaral / chemistry
  • Hydrogen-Ion Concentration
  • Polyvinyl Alcohol / chemistry*
  • Spectroscopy, Fourier Transform Infrared
  • alpha-Amylases / chemistry
  • alpha-Amylases / metabolism*

Substances

  • Aniline Compounds
  • Enzymes, Immobilized
  • polyaniline
  • Polyvinyl Alcohol
  • alpha-Amylases
  • Glutaral