A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP

Qingwang Xue; Lei Wang; Wei Jiang

doi:10.1039/c3cc39064k

A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP

Chem Commun (Camb). 2013 Apr 4;49(26):2640-2. doi: 10.1039/c3cc39064k.

Authors

Qingwang Xue¹, Lei Wang, Wei Jiang

Affiliation

¹ Key Laboratory for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, PR China.

PMID: 23431564
DOI: 10.1039/c3cc39064k

Abstract

A novel label-free fluorescent sensing scheme for sensitive and selective detection of NAD(+) and ATP has been developed based on dumbbell probe-mediated rolling circle amplification (D-RCA)-responsive G-quadruplex formation. This approach can detect 0.5 pM for ATP and 100 fM for NAD(+), much lower than those of previously reported biosensors, and exhibits high discrimination ability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases / chemistry
Adenosine Triphosphatases / metabolism*
G-Quadruplexes*
NAD / chemistry
NAD / metabolism*
Nucleic Acid Amplification Techniques / methods*
Polymerization

Substances

NAD
Adenosine Triphosphatases