The effects of nitrogen (N) fertilization at the rates of 0 and 230 kg hm(-2) combined with selected sulfur (S) fertilization rates of 0, 46, 56, 77, and 115 kg hm(-2) on A- and B-wheat starch granule accumulation, composition, and thermal and pasting properties were investigated. Scanning electron micrographs revealed that A- and B-starch granules accumulation during grain filling can be influenced significantly by N and S fertilization, and B-granules tend to be more sensitive to S fertilization than A-starch granules. The doses of nitrogen and sulfur fertilization on starch properties were not positively correlated; higher concentration of fertilizers failed to cause a higher effects. N230S46 and N230S56 treatment resulted in the higher peak viscosity, trough viscosity, breakdown viscosity, final and setback viscosity, and gelatinization temperature (To, Tp, Tc) according to differential scanning calorimeter (DSC) and rapid visco analyzer (RVA), respectively.