Epitaxial In2O3 films with embedded Fe3O4 oriented nanocolumns were deposited by pulsed laser deposition (PLD) on Y-stabilized ZrO2 (111) substrates. The resulting films show ferromagnetism at room temperature with strong magnetic anisotropy, which can be attributed to the presence of Fe3O4 nanocolumns. Magnetotransport measurements demonstrate a transition from negative magnetoresistance to positive magnetoresistance as the measuring temperature increases. Quantitative coincidence between the anomalous Hall resistivity as a function of the magnetic field and the magnetic hysteresis loop is observed at room temperature, indicating the spin polarization nature of carriers.