Simultaneous determination of cytosine arabinoside and its metabolite, uracil arabinoside, in human plasma using hydrophilic interaction liquid chromatography with UV detection

Masanobu Uchiyama; Yasushi Takamatsu; Kentaro Ogata; Taichi Matsumoto; Shiro Jimi; Kazuo Tamura; Shuuji Hara

doi:10.1002/bmc.2876

Simultaneous determination of cytosine arabinoside and its metabolite, uracil arabinoside, in human plasma using hydrophilic interaction liquid chromatography with UV detection

Biomed Chromatogr. 2013 Jul;27(7):818-20. doi: 10.1002/bmc.2876. Epub 2013 Feb 19.

Authors

Masanobu Uchiyama¹, Yasushi Takamatsu, Kentaro Ogata, Taichi Matsumoto, Shiro Jimi, Kazuo Tamura, Shuuji Hara

Affiliation

¹ Department of Pharmacy, Fukuoka University Hospital, Fukuoka, Japan.

PMID: 23420764
DOI: 10.1002/bmc.2876

Abstract

A practical high-performance liquid chromatography using a Cosmosil HILIC column and UV detection was developed for determining the concentrations of cytosine arabinoside (Ara-C) and uracil arabinoside (Ara-U), which is a major metabolite of Ara-C, in human plasma. This method was used to determine the plasma concentrations of Ara-C and Ara-U in a patient treated with high-dose Ara-C therapy for end-stage renal failure.

MeSH terms

Arabinofuranosyluracil / blood*
Arabinofuranosyluracil / chemistry
Chromatography, High Pressure Liquid / methods*
Cytarabine / blood*
Cytarabine / chemistry
Humans
Hydrophobic and Hydrophilic Interactions
Limit of Detection
Linear Models
Male
Middle Aged
Reproducibility of Results
Spectrophotometry, Ultraviolet

Substances

Cytarabine
Arabinofuranosyluracil