Green synthesis of nanocomposites consisting of silver and protease alpha chymotrypsin

Abstract

The synergy of ultrasonication and the exposure to light radiation was found to be necessary in the formation of nanocomposites of silver and a protease alpha chymotrypsin. The reaction was carried out in aqueous medium and the process took just less than 35 min. Ultrasonication alone formed very negligible number of nanoparticles of <100 nm size whereas light alone produced enough number but the size of the particles was >100 nm. The effects of pH (in the range of 3-5, 9-10), ultrasonication time periods (0-30 min), ultrasonication intensity (33-83 W cm(-2)), energy of light radiation (short UV, long UV and Fluorescent light) and time period of exposure (5-60 min) to different light radiations were studied. The formation of nanocomposites under these effects was followed by surface plasmon resonance (SPR) spectra, dynamic light scattering (DLS), transmission electron microscopy (TEM). Ag-chymotrypsin nanocomposites of sizes ranging from 13 to 72 nm were formed using the synergy of ultrasonication and exposure to short UV radiation. Results show that ultrasonication promoted nuclei formation, growth and reduction of polydispersity by Ostwald ripening.