The objective was to investigate the effects of high-velocity strength training on isometric strength of the leg extensors and jump height in female and male adolescents. Twenty-eight students (13 boys, 15 girls) ages 16 to 17 years participated in this study and were assigned to either a strength training group or a control group. Strength training was conducted over 8 weeks (2 times per week). Pre- and post-training tests included the measurements of maximal isometric force and rate of force development of the leg extensors as well as countermovement jump height. Both girls (effect size = 1.37) and boys (effect size = 0.61) showed significant improvements in jump height. However, significant increases in maximal isometric force (effect size = 1.85) and rate of force development (effect size = 2.23) were found only in girls. In female and male adolescents, high-velocity strength training is an effective training regimen that produced improvements in countermovement jump height in both sexes but higher gains in maximal isometric force and rate of force development in girls.