Abstract
We present the efficient and stable encapsulation of doxorubicin within pH sensitive polymeric vesicles (polymersomes) for intracellular and nuclear delivery to melanoma cells. We demonstrate that PMPC25-PDPA70 polymersomes can encapsulate doxorubicin for long periods of time without significant drug release. We demonstrate that empty polymersomes are non-toxic and that they are quickly and more efficiently internalised by melanoma cells compared to healthy cells. Encapsulated doxorubicin has a strong cytotoxic effect on both healthy and cancerous cells, but when encapsulated it had a preferential effect on melanoma cells indicating that this formulation can be used to achieve an enhanced drug delivery to cancerous cells rather than to the healthy surrounding cells.
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibiotics, Antineoplastic / administration & dosage*
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Antibiotics, Antineoplastic / chemistry
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Antibiotics, Antineoplastic / pharmacokinetics
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Cell Membrane Permeability
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Cells, Cultured
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Doxorubicin / administration & dosage*
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Doxorubicin / chemistry
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Doxorubicin / pharmacokinetics
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Drug Delivery Systems / methods*
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Humans
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Melanoma / drug therapy
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Melanoma / metabolism
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Melanoma / pathology
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Methacrylates / administration & dosage*
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Methacrylates / chemistry
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Methacrylates / pharmacokinetics
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Phosphorylcholine / administration & dosage
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Phosphorylcholine / analogs & derivatives*
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Phosphorylcholine / chemistry
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Phosphorylcholine / pharmacokinetics
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Polymers / administration & dosage*
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Polymers / chemistry
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Polymethacrylic Acids / administration & dosage*
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Polymethacrylic Acids / chemistry
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Polymethacrylic Acids / pharmacokinetics
Substances
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Antibiotics, Antineoplastic
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Methacrylates
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Polymers
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Polymethacrylic Acids
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poly(2-(diisopropylamino)ethyl methacrylate)
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poly(2-methacryloyloxyethyl-phosphorylcholine)
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Phosphorylcholine
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Doxorubicin