Effect of cold storage on total phenolics content, antioxidant activity and vitamin C level of selected potato clones

J Sci Food Agric. 2013 Aug 15;93(10):2437-44. doi: 10.1002/jsfa.6053. Epub 2013 Feb 12.

Abstract

Background: Twelve Colorado-grown specialty potato clones were evaluated for total phenolic content, antioxidant activity and ascorbic acid content at harvest and after 2, 4, 6 and 7 months cold storage at 4 °C. Potato clones were categorized as pigmented ('CO97226-2R/R', 'CO99364-3R/R', 'CO97215-2P/P', 'CO97216-3P/P', 'CO97227-2P/P', 'CO97222-1R/R', 'Purple Majesty', 'Mountain Rose' and 'All Blue'), yellow ('Yukon Gold') and white fleshed ('Russet Nugget', 'Russet Burbank'). Folin-Ciocalteu reagent was used to estimate total phenolic content, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS(•+) ) and 2,2-diphenyl-1-picrylhydrazyl (DPPH(•) ) radical scavenging assays were used to estimate antioxidant capacity.

Results: Pigmented potato genotypes had significantly higher total phenolic content and antioxidant activity at all data points than yellow- and white-fleshed cultivars. Vitamin C content was higher in 'Yukon Gold' than in the other clones. The highest level of vitamin C in all clones was at harvest and after 2 months in cold storage. Vitamin C content in all potato clones dropped rapidly with longer intervals of cold storage. Although total phenolic content and antioxidant activity fluctuated during cold storage, after 7 months of cold storage their levels were slightly higher than at harvest. Total phenolic content was better correlated with Trolox equivalent antioxidant capacity (TEAC)/ABTS(•+) than the TEAC/DPPH(•) radical scavenging assay.

Conclusion: Pigmented potato clones had significantly higher total phenolic content and antioxidant activity, while the yellow-fleshed potato cultivar 'Yukon Gold' had significantly higher vitamin C content. Vitamin C content decreased in all potato clones during cold storage, while total phenolics increased in pigmented clones.

MeSH terms

  • Antioxidants / metabolism*
  • Antioxidants / pharmacology
  • Ascorbic Acid / metabolism*
  • Benzothiazoles / metabolism
  • Biphenyl Compounds / metabolism
  • Cold Temperature*
  • Diet
  • Food Storage / methods*
  • Humans
  • Phenols / metabolism*
  • Picrates / metabolism
  • Pigments, Biological
  • Plant Tubers / metabolism*
  • Solanum tuberosum / classification
  • Solanum tuberosum / metabolism*
  • Species Specificity
  • Sulfonic Acids / metabolism

Substances

  • Antioxidants
  • Benzothiazoles
  • Biphenyl Compounds
  • Phenols
  • Picrates
  • Pigments, Biological
  • Sulfonic Acids
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • 1,1-diphenyl-2-picrylhydrazyl
  • Ascorbic Acid