Abstract
We evaluated the effectiveness of rhamnogalacturonan II (RG-II)-stimulated bone marrow-derived dendritic cells (BMDCs) vaccination on the induction of antitumor immunity in a mouse lymphoma model using EG7-lymphoma cells expressing ovalbumin (OVA). BMDCs treated with RG-II had an activated phenotype. RG-II induced interleukin (IL)-12, IL-1β, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) production during dendritic cell (DC) maturation. BMDCs stimulated with RG-II facilitate the proliferation of CD8+ T cells. Using BMDCs from the mice deficient in Toll-like receptors (TLRs), we revealed that RG-II activity is dependent on TLR4. RG-II showed a preventive effect of immunization with OVA-pulsed BMDCs against EG7 lymphoma. These results suggested that RG-II expedites the DC-based immune response through the TLR4 signaling pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute-Phase Proteins / metabolism
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Adaptor Proteins, Vesicular Transport / metabolism
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Animals
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Bone Marrow Cells / cytology
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Bone Marrow Cells / drug effects
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CD8-Positive T-Lymphocytes / immunology*
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Carrier Proteins / metabolism
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Cell Differentiation / drug effects
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Cell Nucleus / drug effects
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Cell Nucleus / metabolism
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Cell Proliferation / drug effects
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Cytokines / biosynthesis
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Dendritic Cells / cytology
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Dendritic Cells / drug effects
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Dendritic Cells / enzymology
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Dendritic Cells / immunology*
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Enzyme Activation / drug effects
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Lipopolysaccharide Receptors / metabolism
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Lymphocyte Activation / drug effects*
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mitogen-Activated Protein Kinases / metabolism
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Myeloid Differentiation Factor 88 / metabolism
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NF-kappa B / metabolism
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Neoplasms / immunology
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Neoplasms / pathology*
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Pectins / pharmacology*
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Phenotype
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Protein Transport / drug effects
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Receptors, Chemokine / metabolism
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Signal Transduction / drug effects
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T-Lymphocytes, Cytotoxic / cytology
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T-Lymphocytes, Cytotoxic / drug effects
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Toll-Like Receptor 4 / agonists*
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Toll-Like Receptor 4 / metabolism
Substances
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Acute-Phase Proteins
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Adaptor Proteins, Vesicular Transport
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Carrier Proteins
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Cytokines
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Lipopolysaccharide Receptors
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Membrane Glycoproteins
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Myeloid Differentiation Factor 88
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NF-kappa B
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Receptors, Chemokine
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TICAM-1 protein, mouse
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Toll-Like Receptor 4
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lipopolysaccharide-binding protein
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rhamnogalacturonan II
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Pectins
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Mitogen-Activated Protein Kinases