Isolation of the putative biosynthetic gene cluster of 1-deoxynojirimycin by Bacillus amyloliquefaciens 140N, its production and application to the fermentation of soybean paste

Biosci Biotechnol Biochem. 2013;77(2):398-401. doi: 10.1271/bbb.120753. Epub 2013 Feb 7.

Abstract

The 1-deoxynojirimycin (DNJ) biosynthetic gene cluster of Bacillus amyloliquefaciens 140N isolated from traditional Korean fermented food was isolated by PCR screening. It showed 78.9% inhibitory activity against α-glucosidase and produced 0.8 g/L of DNJ in an optimized medium containing 2% soluble starch, 1% tryptone, 0.05% KH(2)PO(4), and 0.05% (NH(2))(4)SO(4). Soybean paste fermented with B. amyloliquefaciens 140N produced DNJ with 84.4% inhibitory activity.

MeSH terms

  • 1-Deoxynojirimycin / metabolism*
  • Bacillus / genetics
  • Bacillus / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Fermentation
  • Food Microbiology
  • Glycine max / metabolism
  • Glycoside Hydrolase Inhibitors*
  • Multigene Family
  • alpha-Glucosidases / metabolism

Substances

  • Bacterial Proteins
  • Glycoside Hydrolase Inhibitors
  • 1-Deoxynojirimycin
  • alpha-Glucosidases