Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs

J Vet Sci. 2013;14(1):95-8. doi: 10.4142/jvs.2013.14.1.95. Epub 2013 Feb 5.

Abstract

There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized only AHSV-3. In contrast, the one selected with AHSV-8 captured by IgG against AHSV-3 recognized all nine AHSV serotypes but not the Bryanston strain of equine encephalosis virus. Serving as evidence for its serogroup-specificity. These two scFvs can help to rapidly confirm the presence of AHSV while additional serotype-specific scFvs may simplify AHSV serotyping.

Keywords: African horsesickness virus; double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA); phage-displayed scFv; serotype-specific.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • African Horse Sickness Virus / isolation & purification*
  • Animals
  • Antibodies, Immobilized
  • Antibodies, Viral / immunology*
  • Chickens
  • Chlorocebus aethiops
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Immunoglobulin G
  • Peptide Library*
  • Serologic Tests / methods
  • Serologic Tests / veterinary
  • Serotyping
  • Single-Chain Antibodies / immunology*
  • Vero Cells

Substances

  • Antibodies, Immobilized
  • Antibodies, Viral
  • Immunoglobulin G
  • Peptide Library
  • Single-Chain Antibodies