The complete mitochondrial genome of a Korean Ussurian tube-nosed bat, Murina ussuriensis, was determined. The mitochondrial genome of M. ussuriensis is 17,285 bp in length with a total base composition of 33.8% A, 28.9% T, 23.8% C, and 13.4% G. All the protein-coding genes (total length of 11,401 bp) were encoded in H-strand except for ND6 in L-strand. The total length of 22 tRNA genes was 1516 bp varying from 62 bp (tRNA (Ser) ((AGY))) to 74 bp (tRNA (Leu) ((UUR)) and tRNA (Gln)). The 12S rRNA and 16S rRNA genes were 972 and 1558 bp in length, respectively. The D-loop region was 2496 bp in length.