Abstract
Flower scent is a composite character determined by a complex mixture of low-molecular-weight volatile molecules. Despite the importance of floral fragrance, our knowledge on factors regulating these pathways remains sketchy. Virus-induced gene silencing (VIGS) and virus-aided gene expression (VAGE) are characterized by a simple inoculation procedure and rapid results as compared to transgenesis, allowing screening and characterization of scent-related genes. Here, we describe methods using TRV as a VIGS/VAGE vector for the characterization of scent-related genes, protein compartmentalization studies, and protein subcellular targeting.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agrobacterium tumefaciens / virology
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Base Sequence
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Flowers / genetics*
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Flowers / metabolism
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Flowers / virology
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Gene Expression
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Gene Knockdown Techniques / methods*
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Genes, Plant
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Genetic Vectors
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Nicotiana / virology
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Petunia / genetics*
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Petunia / metabolism
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Petunia / virology
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / metabolism
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Plant Viruses / genetics*
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RNA Interference*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Volatile Organic Compounds / chemistry
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Volatile Organic Compounds / isolation & purification
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Volatile Organic Compounds / metabolism
Substances
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Plant Extracts
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Recombinant Fusion Proteins
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Volatile Organic Compounds