Directed evolution of a synthetic RNA-protein module to create a new translational switch

Tomoaki Hara; Hirohide Saito; Tan Inoue

doi:10.1039/c3cc38688k

Directed evolution of a synthetic RNA-protein module to create a new translational switch

Chem Commun (Camb). 2013 May 10;49(37):3833-5. doi: 10.1039/c3cc38688k.

Authors

Tomoaki Hara¹, Hirohide Saito, Tan Inoue

Affiliation

¹ Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Kyoto, 606-8502, Japan.

PMID: 23381780
DOI: 10.1039/c3cc38688k

Abstract

A synthetic RNP-binding module was developed to produce an alternative translational switch: an in vitro selection experiment targeting a derivative of L7Ae protein (L7KK) identified a new H23 RNA aptamer that binds tightly to both L7KK and L7Ae. The switch serves as a translational OFF switch for constructing a NOR gate.

MeSH terms

Aptamers, Nucleotide / genetics
Aptamers, Nucleotide / metabolism*
Archaeal Proteins / genetics
Archaeal Proteins / metabolism*
Binding Sites
Electrophoretic Mobility Shift Assay
Genetic Engineering
HeLa Cells
Humans
Models, Molecular
Mutagenesis, Site-Directed
Nucleic Acid Conformation
Protein Binding
Protein Biosynthesis*
RNA, Archaeal / genetics
RNA, Archaeal / metabolism*

Substances

Aptamers, Nucleotide
Archaeal Proteins
RNA, Archaeal