Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry

Eefje de Jong; Arjan S de Jong; Nathalie Smidts-van den Berg; Rob J Rentenaar

doi:10.1016/j.diagmicrobio.2012.12.009

Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry

Diagn Microbiol Infect Dis. 2013 Apr;75(4):431-3. doi: 10.1016/j.diagmicrobio.2012.12.009. Epub 2013 Jan 30.

Authors

Eefje de Jong¹, Arjan S de Jong, Nathalie Smidts-van den Berg, Rob J Rentenaar

Affiliation

¹ Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands.

PMID: 23375086
DOI: 10.1016/j.diagmicrobio.2012.12.009

Abstract

Ninety-nine clinical isolates previously identified as Klebsiella oxytoca were evaluated using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight isolates were identified as Raoultella spp., being 5 Raoultella spp. and 3 K. oxytoca, by 16S rRNA sequencing. These isolates were correctly identified by applying the 10% differential rule for the MALDI-TOF MS score values. This approach might be useful to discriminate Raoultella species from K. oxytoca.

Publication types

Evaluation Study

MeSH terms

Bacteriological Techniques / methods*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Enterobacteriaceae / chemistry*
Enterobacteriaceae / classification*
Humans
Molecular Sequence Data
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S

Associated data

GENBANK/JX102650
GENBANK/JX102651
GENBANK/JX102652
GENBANK/JX102653
GENBANK/JX102654