Radical scavenging capacity of methanolic Phillyrea latifolia L. extract: anthocyanin and phenolic acids composition of fruits

Molecules. 2013 Jan 30;18(2):1798-810. doi: 10.3390/molecules18021798.

Abstract

Radical scavenging capacity of a crude methanolic extract from the fruits of Phillyrea latifolia L., commonly known as green olive tree or mock privet, was investigated with reference to anthocyanin standards, as flavonoids, and phenolic acid standards, as phenylpropanoids. Characterization with high performance liquid chromatography-diode array detection (HPLC-DAD) indicated the presence of keracyanin, kuromanin, cyanidin, ferulic acid, caffeic acid and rosmarinic acid at amounts of 289.1, 90.4, 191.4, 225.2, 221.2 and 190.1 mg/100 g fresh weight (FW) of fruits, respectively. Chlorogenic and p-coumaric acids were found to exist in lower amounts. Trolox equivalent antioxidant capacity (TEAC) and IC(50) values of the plant extract were found to be 1.8 mM Trolox equivalents (TE)/g FW of fruits and 69.4 µg/mL, respectively, indicating the close radical scavenging activity of the extract to those of keracyanin and p-coumaric acid. The crude methanolic P. latifolia L. fruit extract was seen to be fairly potent in radical scavenging. Total phenolic content (TPC) of the plant extract was found to be 1652.9 mg gallic acid equivalent (GAE)/100 g FW of fruits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthocyanins / analysis*
  • Anthocyanins / chemistry
  • Benzothiazoles / metabolism
  • Biphenyl Compounds / metabolism
  • Chromatography, High Pressure Liquid
  • Free Radical Scavengers / pharmacology*
  • Fruit / chemistry*
  • Hydroxybenzoates / analysis*
  • Hydroxybenzoates / chemistry
  • Methanol / chemistry*
  • Olea / chemistry*
  • Picrates / metabolism
  • Plant Extracts / pharmacology*
  • Regression Analysis
  • Sulfonic Acids / metabolism

Substances

  • Anthocyanins
  • Benzothiazoles
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Hydroxybenzoates
  • Picrates
  • Plant Extracts
  • Sulfonic Acids
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • 1,1-diphenyl-2-picrylhydrazyl
  • phenolic acid
  • Methanol