Sequencing-based genotyping of mixed human papillomavirus infections by use of RipSeq software

J Clin Microbiol. 2013 Apr;51(4):1278-80. doi: 10.1128/JCM.00091-13. Epub 2013 Jan 30.

Abstract

Sequencing-based pathogen identification directly from clinical specimens requires time-consuming interpretation, especially with mixed chromatograms when multiple microorganisms are detected. We assessed RipSeq Mixed software for human papillomavirus (HPV) genotyping by comparison to the linear array HPV genotyping assay. RipSeq Mixed provided rapid, sequencing-based HPV typing for single-type infections and coinfections with 2 types.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coinfection / virology*
  • Computational Biology / methods*
  • DNA, Viral / chemistry*
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Humans
  • Papillomaviridae / classification*
  • Papillomaviridae / genetics*
  • Papillomaviridae / isolation & purification
  • Papillomavirus Infections / virology*
  • Sequence Analysis, DNA / methods*
  • Software

Substances

  • DNA, Viral