Objective: To investigate the dys-psychological stress effect on the growth of subcutaneous xenotransplanted tumor in nude mice bearing human epithelium ovarian carcinoma, and the influence on P53 and NFκBp65 expressions.
Methods: The subcutaneous tumor xenografts were established by implanting human epithelium ovarian carcinoma tissues into nude mice and the dys-psychological stress model was established with restraint. The mice were randomized into the following four treatment groups with each group six mice respectively: tumor group (group A), normal saline intraperitoneal injection; tumor with stress group (group B), normal saline intraperitoneal injection; tumor therapy group (group C), cisplatin intraperitoneal injection; and tumor therapy with stress group (group D), cisplatin intraperitoneal injection. The expressions of P53 and NFκBp65 in tumor tissues were determined by Western blotting.
Results: The expressions of P53 and NFκBp65 in each restraint group were enhanced compared with the control groups (P<0.05).
Conclusion: The dys-psychological stress may induce the high expressions of P53 and NFκBp65 proteins and further promote tumor growth.
Keywords: Constraint stress; Oncoprotein; Ovarian cancer animal model.