Hypertrophic cardiomyopathy is defined as a primary and familial cardiac disorder characterized by heterogeneous expression, unique pathophysiology and considerable diversity in clinical presentation. Clinical diagnosis was mainly based on the performance of ECG. In addition, cardiovascular MRI or ECG plays an important role in the diagnosis. Nevertheless, myocardial radionuclide imaging, which could provide detailed information on myocardial perfusion, metabolism and neurological function, is a valuable method for exploring the inter-relationship between the morphological, pathophysiological and functional changes in hypertrophic cardiomyopathy. It is also helpful in evaluating the effect of transcoronary ethanol septal ablation and prognosis in patients with hypertrophic cardiomyopathy, which suggests that it is an important imaging method in the comprehensive evaluation of hypertrophic cardiomyopathy.